Ultima 2Pplus - System specifications

*Ultima 2Pplus*

Ultima 2Pplus

Laser input

  • Coherent Chameleon Discovery NX TPC dual-output femtosecond laser

    • Widely tunable from 660 to 13200 nm

    • Fixed secondary output at ~1040 nm (>2.8 W)

    • Peak power >2.7 W

  • Laser power meter with pick-off window for monitoring

  • Integrated AOM modules for imaging and uncaging pathways

  • Switchable IR mirror mechanics for routing between galvo and resonant scanning paths

Laser scanner

  • Dual independent sets of X-Y galvanometer mirrors for standard

    • Standard set for raster scan imaging

    • Second set for precise, programmable point scanning and photostimulation

  • Resonant scanner (8 kHz resonant galvo for up to 30 fps)

  • Field of view:

    • Galvo scanning: 22.7 mm field number (1.11 × 1.11 mm with 16× 0.8 NA objective)

    • Overscan mode: 28 mm field number (1.4 × 1.4 mm with 16× 0.8 NA objective)

  • Scan modes: frame scanning, ROI scanning, freehand line scanning, scan rotation, optical zoom

Detectors

  • Two high-efficiency Hamamatsu GaAsP PMTs with mounting flanges

  • Pre-amplifier and HV controller optimized for GaAsP tubes

  • Close-proximity detector design for maximum photon collection with high NA objectives

  • Dual emission filters: ET525/70m-2p and ET595/50m-2p with t565lpxr dichroic beam splitter

  • 2″ collection optics for maximizing photon throughput

Optics and nosepiece

  • Single objective nosepiece (M32 threading) with rotational axis for angled imaging (±87°)

  • Motorized Z-focus with reliable 250 nm step size (electronics capable of 100 nm increments)

  • Motorized pitch nosepiece for precise rotational control

  • Beam expander lens set (3×) for adapting to different objective back apertures

  • Objectives supported

    • Nikon CFI Plan Apo Lambda 4×

    • Nikon CFI75 16×/0.8 NA

    • Nikon Apo 60× Water/1.0 NA

Epi-fluorescence imaging

  • Modified epi-illuminator arm with integrated LED excitation source

  • Widefield fluorescence imaging for sample positioning and overview imaging

  • Integrated CMOS camera for capturing epifluorescence images

Imaging chamber and enclosure

  • Light-tight Faraday cage enclosure (43.25″ × 36″ × 40″) with interlocked laser safety ports

  • Table laser safety covers (for 4′ × 8′ table)

Electronics

  • FPGA-based control electronics for precise synchronization

  • Multi-channel analog integration with 12-bit A/D conversion

  • Pre-amplifier and HV electronics tailored for GaAsP detectors

  • Remote controller for motorized Z-axis

Software

  • Prairie View scan control and acquisition software

  • Simultaneous acquisition from up to four channels with independent LUTs

  • Time Series acquisition for flexible experimental design

  • Resonant Galvo+ software module for high-speed imaging

  • Integrated calibration and drift compensation for ETL remote focusing modules

Available accessories

Operational protocols and risk assessments

The SOPs and RAs related to the use of the Ultima 2Pplus system are available https://drive.google.com/drive/u/0/folders/1biXgabX9BQkMIouT0Lx4Yg9P1y6UJ8bb, upon request. These documents are stored in the designated folder and include:

  • SOP for Ultima 2Pplus utilization

  • RA for Ultima 2Pplus with anesthesia