IVIS Spectrum - System specifications

*IVIS Spectrum*

IVIS Spectrum

CCD camera

  • Back-thinned, back-illuminated Grade 1 CCD sensor

  • Thermoelectric cooling to -90°C for low dark current and noise

  • Pixel size: 13.5 µm

  • Minimum pixel resolution: 20 µm

  • Sensor dimensions: 2048 × 2048 pixels

  • Maximum sensor binning: 16

  • Quantum efficiency: 85% in the 500–700 nm range and 30% in the 400–900 nm range

Lens

  • 6-inch diameter optics

  • Numerical aperture adjustable from f/1 to f/8

  • Discrete magnifications: 1.5×, 2.5×, 5×, and 8.7×

  • Corresponding fields of view: 3.9 cm to 22.5 cm

  • High-resolution imaging down to 20 µm with small field of view

Optical path

  • High-throughput imaging with a large field of view (up to 5 mice or 2 rats simultaneously)

  • 10 high-efficiency narrow bandpass excitation filters (415–760 nm, 30 nm bandwidth)

  • 18 high-efficiency narrow bandpass emission filters (490–850 nm, 20 nm bandwidth)

  • Optical switch enabling both epi-illumination and trans-illumination fluorescence modes

Imaging chamber

  • Temperature-controlled platform for maintaining physiological temperature during imaging

  • Integrated RAS-4 gas anesthesia system

  • light tight chamber

Software

  • Radiometric calibration of measurements in radiance (bioluminescence) and radiance efficiency (fluorescence) for reproducible results

  • Spectral unmixing for separating multiple fluorescent or bioluminescent signals and background subtraction

  • Co-registration with anatomical atlases or CT/MRI for overlaying structural and functional data

  • 3D bioluminescence and fluorescence tomography with depth estimation and quantitative analysis

  • Exportation of DICOM image format for 3D co-registration

Available accessories

  • Fluorescence and bioluminescence phantom mouse for calibration, validation, quality control

  • Mouse Imaging Shuttle (MIS) for 3D multimodal co-registration with microCT or MRI systems

Operational protocols and risk assessments

The SOPs and RAs related to the use of the IVIS Spectrum system are available https://drive.google.com/drive/u/1/folders/1yU5AkpK4jt7LfB_xHxhKC4837ZLwxuR2, upon request. These documents are stored in the designated folder and include:

  • IVIS Spectrum user manual

  • SOP for IVIS Spectrum utilization

  • SOP for RAS-4 rodent anesthesia system

  • RA for IVIS Spectrum with anesthesia

  • Revvity useful information