.. _IVIS-specifications:

IVIS Spectrum - System specifications
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   :alt: *IVIS Spectrum*
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*IVIS Spectrum*

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CCD camera
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- Back-thinned, back-illuminated Grade 1 CCD sensor
- Thermoelectric cooling to -90°C for low dark current and noise
- Pixel size: 13.5 µm
- Minimum pixel resolution: 20 µm
- Sensor dimensions: 2048 × 2048 pixels
- Maximum sensor binning: 16
- Quantum efficiency: 85% in the 500–700 nm range and 30% in the 400–900 nm range

Lens
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- 6-inch diameter optics
- Numerical aperture adjustable from f/1 to f/8
- Discrete magnifications: 1.5×, 2.5×, 5×, and 8.7×
- Corresponding fields of view: 3.9 cm to 22.5 cm
- High-resolution imaging down to 20 µm with small field of view

Optical path
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- High-throughput imaging with a large field of view (up to 5 mice or 2 rats simultaneously)
- 10 high-efficiency narrow bandpass excitation filters (415–760 nm, 30 nm bandwidth)
- 18 high-efficiency narrow bandpass emission filters (490–850 nm, 20 nm bandwidth)
- Optical switch enabling both epi-illumination and trans-illumination fluorescence modes

Imaging chamber
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- Temperature-controlled platform for maintaining physiological temperature during imaging
- Integrated RAS-4 gas anesthesia system
- light tight chamber

Software
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- Radiometric calibration of measurements in radiance (bioluminescence) and radiance efficiency (fluorescence) for reproducible results
- Spectral unmixing for separating multiple fluorescent or bioluminescent signals and background subtraction
- Co-registration with anatomical atlases or CT/MRI for overlaying structural and functional data
- 3D bioluminescence and fluorescence tomography with depth estimation and quantitative analysis
- Exportation of DICOM image format for 3D co-registration

Available accessories
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- Fluorescence and bioluminescence phantom mouse for calibration, validation, quality control
- Mouse Imaging Shuttle (MIS) for 3D multimodal co-registration with microCT or MRI systems

Operational protocols and risk assessments
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The SOPs and RAs related to the use of the IVIS Spectrum system are available https://drive.google.com/drive/u/1/folders/1yU5AkpK4jt7LfB_xHxhKC4837ZLwxuR2,
upon request. These documents are stored in the designated folder and include:

- IVIS Spectrum user manual
- SOP for IVIS Spectrum utilization
- SOP for RAS-4 rodent anesthesia system
- RA for IVIS Spectrum with anesthesia
- Revvity useful information