nVue-nVision - System specifications

*nVue-nVision*

nVue-nVision

nVue

Camera

  • Sensor format: 1280 × 800 pixels

  • Frame rate: 6-60 FPS in standard mode and 100 FPS in fast sensor mode (up to 30 FPS in multiplex mode)

  • Electronic focus: range of 300 µm with ~0.3 µm steps

Optics and lens

  • Objective lens NA: 0.5

  • Field of view: 1050 × 650 µm

  • Excitation wavelengths:

    • Green channel: 455–480 nm

    • Red channel: 545–580 nm

  • Emission wavelengths:

    • Green channel: 499–530 nm

    • Red channel: 600–660 nm

  • Channel excitation Irradiance: 0–2 mW/mm²

  • Resolution: 0.1 mW/mm² at the objective front surface

Available accessories

Lens

  • ProView Integrated Lens 1.0mm x 4.0mm

  • ProView Integrated Prism Lens 1.0 x 4.3mm

  • ProView DC Integrated Lens 0.66 x 7.5mm

  • ProView Integrated Lens 0.6 x 7.3mm

  • ProView Integrated Lens 0.5 x 8.4mm

  • ProView Practice Probes 1.0 x 4.0 mm (training)

  • ProView Practice Probes 0.6 x 7.2 mm (training)

Viral vectors

  • Ready-to-Image virus for mouse neocortex - AAV1.Camk2a.GCaMP6f.WPRE.bGHp

  • Ready-to-Image Virus for mouse dorsal CA1 hippocampus - AAV1.Camk2a.GCaMP6f.WPRE.bGHp

nVision

Camera

  • Sensor type: CMOS, color

  • Sensor size: 5.63 × 3.17 mm

  • Sensor format: Up to 1920 × 1080 pixels (Full HD)

  • Frame rate: Up to 30 FPS

  • Real-time animal tracking

Lens

  • Focal length: 2.8 – 12 mm

  • Near-infrared filter: cut-on >850 nm and transmittance average > 80% in 850–2200 nm range

Available accessories

  • Extra camera and near-infra red filter

Commutator

Functionality and compatibility

  • Type: Active motorized commutator

  • Microscope Compatibility: nVista, nVoke, nVue

  • Animal compatibility: mouse, rat

  • Rotation freedom: 360°

Performance

  • Static torque: <100 μNm

  • Video data rate: up to 1.4 Gbps

  • Max rotation speed: 30 rpm

Software suite

  • Inscopix Data Acquisition Software IDAS - data acquisition platform and automated synchronization of nVue and nVision

  • Inscopix Data Processing Software IDPS - pre-processing pipeline

  • Inscopix Data Exploration, Analysis & Sharing Software IDEAS - Data analysis & interpretation

Operational protocols and risk assessments

The SOPs and RAs related to the use of the nVue-nVision platform are available https://drive.google.com/drive/u/1/folders/1_Y-os1suhyrXSIISQ3GZnKq0_TVdfHoT, upon request. These documents are stored in the designated folder and include:

  • nVue user manual

  • nVision user manual

  • commutator user manual

  • IDPS user manual

  • SkyScan 1276 workflow

  • SOP for nVue-nVision utilization

  • RA for nVue-nVision

  • RA for intracranial vector injection

  • RA for intracranial lens implantation

  • RA for intracardia perfusion

  • Inscopix IQ useful information