.. _nVue-specifications:

nVue-nVision - System specifications
====================================

.. image:: ../_static/nVue-nVision.jpg
   :alt: *nVue-nVision*
   :width: 1000px
   :align: center

*nVue-nVision*

nVue
----

Camera
^^^^^^
- Sensor format: 1280 × 800 pixels
- Frame rate: 6-60 FPS in standard mode and 100 FPS in fast sensor mode (up to 30 FPS in multiplex mode)
- Electronic focus: range of 300 µm with ~0.3 µm steps

Optics and lens
^^^^^^^^^^^^^^^
- Objective lens NA: 0.5
- Field of view: 1050 × 650 µm
- Excitation wavelengths:
    - Green channel: 455–480 nm
    - Red channel: 545–580 nm
- Emission wavelengths:
    - Green channel: 499–530 nm
    - Red channel: 600–660 nm
- Channel excitation Irradiance: 0–2 mW/mm²
- Resolution: 0.1 mW/mm² at the objective front surface

Available accessories
^^^^^^^^^^^^^^^^^^^^^

Lens
""""
- ProView Integrated Lens 1.0mm x 4.0mm
- ProView Integrated Prism Lens 1.0 x 4.3mm
- ProView DC Integrated Lens 0.66 x 7.5mm
- ProView Integrated Lens 0.6 x 7.3mm
- ProView Integrated Lens 0.5 x 8.4mm
- ProView Practice Probes 1.0 x 4.0 mm (training)
- ProView Practice Probes 0.6 x 7.2 mm (training)

Viral vectors
"""""""""""""
- Ready-to-Image virus for mouse neocortex - AAV1.Camk2a.GCaMP6f.WPRE.bGHp
- Ready-to-Image Virus for mouse dorsal CA1 hippocampus - AAV1.Camk2a.GCaMP6f.WPRE.bGHp

nVision
-------

Camera
^^^^^^
- Sensor type: CMOS, color
- Sensor size: 5.63 × 3.17 mm
- Sensor format: Up to 1920 × 1080 pixels (Full HD)
- Frame rate: Up to 30 FPS
- Real-time animal tracking

Lens
^^^^
- Focal length: 2.8 – 12 mm
- Near-infrared filter: cut-on >850 nm and transmittance average > 80% in 850–2200 nm range

Available accessories
^^^^^^^^^^^^^^^^^^^^^
- Extra camera and near-infra red filter

Commutator
----------

Functionality and compatibility
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
- Type: Active motorized commutator
- Microscope Compatibility: nVista, nVoke, nVue
- Animal compatibility: mouse, rat
- Rotation freedom: 360°

Performance
^^^^^^^^^^^
- Static torque: <100 μNm
- Video data rate: up to 1.4 Gbps
- Max rotation speed: 30 rpm

Software suite
--------------
- Inscopix Data Acquisition Software IDAS - data acquisition platform and automated synchronization of nVue and nVision
- Inscopix Data Processing Software IDPS - pre-processing pipeline
- Inscopix Data Exploration, Analysis & Sharing Software IDEAS - Data analysis & interpretation

Operational protocols and risk assessments
------------------------------------------
The SOPs and RAs related to the use of the nVue-nVision platform are available https://drive.google.com/drive/u/1/folders/1_Y-os1suhyrXSIISQ3GZnKq0_TVdfHoT,
upon request. These documents are stored in the designated folder and include:

- nVue user manual
- nVision user manual
- commutator user manual
- IDPS user manual
- SkyScan 1276 workflow
- SOP for nVue-nVision utilization
- RA for nVue-nVision
- RA for intracranial vector injection
- RA for intracranial lens implantation
- RA for intracardia perfusion
- Inscopix IQ useful information