.. _sai-scheduling: SAI Study Scheduling ==================== Study general workflow ---------------------- The following outlines the **general workflow** for initiating a standard study using the SAI Core. .. mermaid:: .. raw:: html
.. mermaid:: graph TD %% Styles classDef consult fill:#d9c9eb,stroke:#7148af,stroke-width:1px; classDef exvivo fill:#bfe7d0,stroke:#3a7f5c,stroke-width:1px; classDef invivo fill:#c4ddf5,stroke:#2f5e87,stroke-width:1px; classDef staff fill:#f3e1c7,stroke:#b56b2d,stroke-width:1px; classDef user fill:#f5baf4,stroke:#c441ba,stroke-width:1px; %% Nodes A["Initial Consultation"]:::consult %% Group 1: Ex vivo path B["Ex vivo
Biological Sample
Material Science"]:::exvivo D1["Project Feasibility?"]:::exvivo %% Group 2: In vivo path C["In vivo"]:::invivo L1["Project Feasibility?"]:::invivo M["Vivarium Access?"]:::invivo N["IACUC/IBC Approved?"]:::invivo O["Animals Ready?"]:::invivo %% Group 3: Staff-handled E["Staff-Assisted Imaging"]:::staff F["Project Request Submission"]:::staff G["SOPs Signature"]:::staff H["Calendar Invitation"]:::staff I["Data Acquisition with Maylis"]:::staff J["Data Transfer to SAI Folder"]:::staff K["Data Analysis"]:::staff %% Group 4: User-operated P["User-Operated Imaging"]:::user Q["Project Request Submission"]:::user R["Schedule Training with Maylis"]:::user S["Training Attendance Form"]:::user T["SOPs/RAs Signature"]:::user U["Access to Equipment Booking"]:::user V["Data Acquisition by User"]:::user W["Data Transfer to SAI Folder"]:::user X["Data Analysis"]:::user %% Flow A --> B A --> C %% Ex vivo flow B --> D1 --> E E --> F --> G --> H --> I --> J --> K %% In vivo flow C --> L1 --> M M --✅ Yes --> N N --✅ Yes --> O O --✅ Yes --> E O --✅ Yes --> P %% User-operated flow P --> Q --> R --> S --> T --> U --> V --> W --> X %% Clickable Nodes click A "mailto:maylis.boitet@nyu.edu" click Q "https://forms.gle/d3xRyphopVUj6PwW6" click S "https://docs.google.com/forms/d/e/1FAIpQLScLW1MOvo-9aAwX2_04FcyLGPR9xtDso9hu9SEixUy2VzuAiw/viewform" click U "https://corelabs.abudhabi.nyu.edu/" .. raw:: html
Study initiation ---------------- Initial consultation ^^^^^^^^^^^^^^^^^^^^ To initiate a research project utilizing equipment from the SAI Lab, please contact maylis.boitet@nyu.edu to arrange an **initial consultation** and assess **project feasibility**. The email should include a concise project description and specify the imaging resource intended for use. Project registration ^^^^^^^^^^^^^^^^^^^^ Upon completion of the consultation, please submit the **online project registration** form https://forms.gle/d3xRyphopVUj6PwW6. You will then receive an email containing the relevant Standard Operating Procedures (SOPs) for SAI operations, imaging procedures, and material transfer. These documents must be reviewed and signed during your initial imaging session or training. Training and scheduling ----------------------- The SAI Core offers both **user-operated** and **staff-assisted imaging** services, depending on the type of equipment and the specific requirements of the study. User-operated imaging ^^^^^^^^^^^^^^^^^^^^^ Equipment training """""""""""""""""" Users are required to schedule a **training session** with Maylis Boitet, which will cover equipment utilization, including a technology overview, data acquisition and analysis procedures, and emergency shutdown protocols. Upon completion of the training, users must submit the training attendance request form https://docs.google.com/forms/d/e/1FAIpQLScLW1MOvo-9aAwX2_04FcyLGPR9xtDso9hu9SEixUy2VzuAiw/viewform. Once the completed attendance sheet is received, the corresponding **Standard Operating Procedures (SOPs) and Risk Assessments (RAs)** for equipment use will be shared and must be thoroughly reviewed and signed by the registered individual. Strict adherence to the SOPs is mandatory when operating the equipment to ensure the safety and security of both the operator and the animal during imaging sessions. Equipment scheduling """""""""""""""""""" Once all the required documentation is signed and provided, users will be granted access to the booking system to schedule their imaging sessions https://corelabs.abudhabi.nyu.edu/. SAI resources can be accessed through the "Reservations" or "Schedule" sections, as well as from the upper drop-down menu under "Biomedical Imaging." Prior to using any equipment, users must schedule their session through the CTP booking system and adhere to the SAI scheduling policies. Staff-assisted imaging ^^^^^^^^^^^^^^^^^^^^^^ When staff assistance is required for an imaging study, please send a Google Calendar invitation to maylis.boitet@nyu.edu, ensuring that your name and the imaging modality are clearly stated in the subject line. Before submitting the request, verify the availability of the required resource using the booking system https://corelabs.abudhabi.nyu.edu/. SAI room access ^^^^^^^^^^^^^^^ All users who require access to the SAI room must have prior access to the vivarium as well as an approved IACUC protocol for live animal imaging. Daily equipment scheduling strategy ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ The NYUAD vivarium houses animals with **varying health statuses**, including Specific Pathogen-Free (SPF), conventional, and biohazard categories. To minimize the risk of cross-contamination and maintain the health status integrity of the husbandry environment, an equipment scheduling strategy has been implemented. This strategy prioritizes sample cleanliness and reduces contamination risk by adhering to a defined daily imaging order. Daily Imaging Order (from lowest to highest contamination risk): - SPF vivarium animals or biological samples - Conventional vivarium animals or biological samples - Biohazard vivarium animals or biological samples - External samples (fixed biological specimens, materials science samples) Adherence to this imaging sequence is mandatory to ensure biosafety and preserve the health classification of vivarium environments. Scheduling policies ------------------- Equipment booking ^^^^^^^^^^^^^^^^^ Use of any SAI resource must be scheduled in advance through the designated booking system https://corelabs.abudhabi.nyu.edu/. Users are encouraged to reserve their imaging timeslots at least one week ahead of time. For staff-assisted imaging, a minimum notice of 24 hours is required, while user-operated sessions must be booked with at least 4 hours notice. Short-notice bookings should be avoided to ensure proper planning and support. When reserving equipment, please account for warm-up time and cleaning procedures to ensure optimal performance and adherence to safety protocols. Cancellation ^^^^^^^^^^^^ Users must cancel any scheduled imaging sessions as early as possible to allow reallocation of resources. Cancellations should be made at least 24 hours in advance through the CTP booking system https://corelabs.abudhabi.nyu.edu/. Repeated last-minute cancellations or no-shows may result in temporary suspension of booking privileges. Please notify core staff immediately in the event of unavoidable delays or emergencies. .. _required-documentation: Required documentation ---------------------- All research projects must obtain the requisite regulatory approvals and certifications prior to initiation. Animal protocol (IACUC) ^^^^^^^^^^^^^^^^^^^^^^^ All **live animal imaging studies** must receive prior approval from the **Institutional Animal Care and Use Committee (IACUC)**. If your study involves in vivo imaging, it is recommended that you amend your existing IACUC protocol as early as possible to ensure compliance and avoid delays. The SAI Core is finalizing an IACUC protocol encompassing standard imaging procedures, which, once approved, may be utilized to initiate small pilot studies, provided the proposed procedure falls within the scope of this core protocol. For assistance with incorporating live animal imaging into your IACUC-approved protocol, please contact Dr. Maylis Boitet. For inquiries related to IACUC protocol submission or regulatory matters, please reach out to William Pressley at wdp1@nyu.edu. Biosafety protocol (IBC) ^^^^^^^^^^^^^^^^^^^^^^^^ All research involving the use of **hazardous materials**, including tumor cell lines and adeno-associated viral (AAV) vectors, must obtain prior approval from the **Institutional Biosafety Committee (IBC)** before initiation. For any assistance, please contact Mir Hussain Nawaz hussain@nyu.edu. Radiation safety certificate ^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Researchers seeking access to the microCT scanner must submit a valid **Radiation Safety Certificate** and comply with the NYUAD Radiation Safety Program. The online Radiation Safety Training can be accessed at: https://moodle.abudhabi.nyu.edu/course/view.php?id=72. Laser safety certificate ^^^^^^^^^^^^^^^^^^^^^^^^ Researchers seeking access to the multiphoton fluorescence microscopy must submit a valid ** Laser Safety Course** and comply with the NYUAD Laser Safety Program. The online Radiation Safety Training can be accessed at: - Basic Laser Safety https://moodle.abudhabi.nyu.edu/course/view.php?id=67 - Advanced Laser Safety https://moodle.abudhabi.nyu.edu/course/view.php?id=73